UPRES EA2493, Université de Versailles St-Quentin, PRES Universud Paris, France.
Placenta. 2012 Sep;33(9):677-81. doi: 10.1016/j.placenta.2012.05.011. Epub 2012 Jun 15.
We describe here for the first time the characterization of family member of netrins, netrin-4 and its receptor neogenin, during the development of the placenta. By using western blots and RT-PCR, we demonstrated the presence of netrin-4 and its receptor neogenin protein as well as their transcripts. Using immunohistochemistry, we studied the distribution of netrin-4 and neogenin in both the first trimester and term placenta. We observed staining of netrin-4 in villous and extravillous cytotrophoblasts, syncytiotrophoblast, and endothelial cells whereas staining in stromal cells was faint. In decidua, we observed netrin-4 labelling in glandular epithelial cells, perivascular decidualized cells, and endothelial cells. However, neogenin was absent in villous and extravillous cytotrophoblasts and was expressed only on syncytiotrophoblast and placental stromal cells in the first trimester and at term placenta. The pattern of distribution suggests that a functional netrin-4-neogenin pathway might be restricted to syncytiotrophoblasts, mesenchymal cells, and villous endothelial cells. This pathway function might vary with its localization in the placenta. It is possibly involved in angiogenesis, morphogenesis, and differentiation.
我们首次描述了在胎盘发育过程中，神经导向因子 netrin-4 及其受体 neogenin 的家族成员的特征。通过 Western blot 和 RT-PCR，我们证明了 netrin-4 及其受体 neogenin 蛋白及其转录本的存在。通过免疫组织化学，我们研究了 netrin-4 和 neogenin 在早孕期和足月胎盘中的分布。我们观察到 netrin-4 在绒毛和绒毛外滋养层细胞、合体滋养层和内皮细胞中染色，而在基质细胞中的染色较弱。在蜕膜中，我们观察到 netrin-4 在腺上皮细胞、血管周围蜕膜化细胞和内皮细胞中标记。然而，neogenin 在绒毛和绒毛外滋养层细胞中不存在，仅在早孕期和足月胎盘的合体滋养层和胎盘基质细胞中表达。这种分布模式表明，功能性 netrin-4-neogenin 途径可能仅限于合体滋养层、间质细胞和绒毛内皮细胞。该途径的功能可能因其在胎盘内的定位而异。它可能参与血管生成、形态发生和分化。
