Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
Arch Bronconeumol. 2012 Sep;48(9):316-9. doi: 10.1016/j.arbres.2012.04.022. Epub 2012 Jun 15.
To evaluate the effect of a preparation with bacterial ribosomes and proteoglycans from Klebsiella pneumoniae «R» on the in vitro expression of CD11c and CD103 molecules in neutrophils from peripheral blood.
Isolation of neutrophils from peripheral blood with Ficoll-Paque, incubation with R and detection of CD11c and CD103 through flow cytometry.
Six hours after the incubation period, CD11c expression increased significantly compared with the control with 125 and 500μg/ml of R (P=.017 and P=.006, respectively). CD103 expression induced with 125μg/ml of R after 6hours was significantly higher than that observed after 4hours at the same concentration (P=.014) and that found with 62.5μg/ml (P=.017) of R.
The increased expression of CD11c and CD103 induced by R in the neutrophils could contribute to the R mechanism against respiratory pathogens.
评估一种含有肺炎克雷伯氏菌细菌核糖体和蛋白聚糖的制剂对体外外周血中性粒细胞 CD11c 和 CD103 分子表达的影响。
使用 Ficoll-Paque 从外周血中分离中性粒细胞，用 R 孵育，并通过流式细胞术检测 CD11c 和 CD103。
孵育 6 小时后，与对照相比，125 和 500μg/ml 的 R 显著增加了 CD11c 的表达（P=.017 和 P=.006）。与同一浓度孵育 4 小时相比，用 125μg/ml 的 R 诱导的 CD103 表达在 6 小时时显著更高（P=.014），并且与 62.5μg/ml 的 R 相比也更高（P=.017）。
R 诱导中性粒细胞中 CD11c 和 CD103 的表达增加可能有助于 R 对抗呼吸道病原体的机制。
