Division of Microbiology, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Biochem Biophys Res Commun. 2012 Jul 13;423(4):733-8. doi: 10.1016/j.bbrc.2012.06.027. Epub 2012 Jun 13.
In many bacterial feo loci, the feoA gene is associated with the feoB gene. While the feoB-encoded FeoB protein has been demonstrated as a ferrous iron [Fe(II)] transporter, the function of the feoA gene product, FeoA, is unknown. In the present study, we report that the FeoA protein interacts with the FeoB Fe(II) transporter, which is required for FeoB-mediated Fe(II) uptake in Salmonella enterica. Iron uptake assay revealed that in the absence of FeoA, FeoB import of Fe(II) is impaired. Bacterial two-hybrid assay determined that the FeoA protein directly and specifically binds to the FeoB transporter in vivo. This FeoA-FeoB interaction appeared necessary for FeoB-mediated Fe(II) uptake because Salmonella expressing the mutant FeoA that cannot interact with FeoB failed to uptake Fe(II) via the FeoB transporter. Finally, we showed that the FeoA protein does not affect expression of the FeoB transporter per se.
在许多细菌 feo 基因座中，feoA 基因与 feoB 基因相关联。虽然 feoB 编码的 FeoB 蛋白已被证明是亚铁（Fe(II)）转运蛋白，但 feoA 基因产物 FeoA 的功能尚不清楚。在本研究中，我们报告 FeoA 蛋白与 FeoB Fe(II)转运蛋白相互作用，这对于沙门氏菌中的 FeoB 介导的 Fe(II)摄取是必需的。铁摄取测定表明，在没有 FeoA 的情况下，FeoB 对 Fe(II)的摄取受损。细菌双杂交测定确定 FeoA 蛋白在体内直接且特异性地与 FeoB 转运蛋白结合。这种 FeoA-FeoB 相互作用似乎对于 FeoB 介导的 Fe(II)摄取是必要的，因为表达不能与 FeoB 相互作用的突变 FeoA 的沙门氏菌无法通过 FeoB 转运蛋白摄取 Fe(II)。最后，我们表明 FeoA 蛋白本身并不影响 FeoB 转运蛋白的表达。
