Hazum E, Chang K J, Cuatrecasas P
Science. 1979 Nov 30;206(4422):1077-9. doi: 10.1126/science.227058.
A bioactive, fluorescent derivative of enkephalin, Tyr-D-Ala-Gly-Phe-Leu-Lys-rhodamine, was used to determine the distribution of opiate receptors in living neuroblastoma cells. The receptors appeared in clusters on the cell surface, and no internalization was detected. No specific fluorescence or clusters were observed in the presence of [D-Ala2, Leu5]enkephalin or at 4 degrees C, and the clusters were much reduced under ionic conditions (that is, with 100 millimolars sodium) that specifically decrease the binding of opiate agonists.
一种脑啡肽的生物活性荧光衍生物，即酪氨酸-右旋丙氨酸-甘氨酸-苯丙氨酸-亮氨酸-赖氨酸-罗丹明，被用于确定阿片受体在活神经母细胞瘤细胞中的分布。这些受体出现在细胞表面的簇状结构中，未检测到内化现象。在存在[右旋丙氨酸2，亮氨酸5]脑啡肽的情况下或在4摄氏度时，未观察到特异性荧光或簇状结构，并且在能特异性降低阿片激动剂结合的离子条件下（即100毫摩尔钠存在时），簇状结构明显减少。
