[Inhibition of glucose in Zajdela hepatoma by 6-phosphogluconate; the role of 3-phosphoglycerate].

Influence of 6-phosphogluconate and 3-phosphoglycerate have been studied for their effect on the fructose-6-phosphate glycolytic transformation reactions in homogenates of the Zajdela hepatoma cells. It is established that 6-phosphogluconate inhibits formation of lactate from fructose-6-phosphate and increases the ratio: dioxyacetone-phosphate/lactate. The influence of 6-phosphogluconate on the formation of lactate from the fructose-1,6-bisphosphate is similar. 3-phosphoglycerate removes the effect of 6-phosphogluconate, its content being unchanged in samples, which indicates rather the regulatory, than the substrate role of 3-phosphoglycerate. Analogous experiments with homogenates of the rat liver show that 6-phosphogluconate inhibits hexosephosphate isomerase, but almost all the introduced substrate (fructose-6-phosphate) is transformed into glucose. Processes of fructose-6-phosphate consumption in the hepatoma and liver are opposite.