Department of Neuroscience, Pharmacology section, University of Pisa, Via Roma 55, Pisa, Italy.
Plant Foods Hum Nutr. 2012 Sep;67(3):289-93. doi: 10.1007/s11130-012-0296-y.
The cardioprotective and anti-aging effects of red wine phenols, especially resveratrol (RSV), are well known. One of the most interesting biological properties of RSV and other naturally occurring phenols is the regulation of the expression and activity of SIRT1 (silent mating type information regulation 2 homolog). In view of the role of SIRT1 in acute and chronic renal diseases, we decided to study the effects of RSV-poor red wines on the expression of SIRT1 and HIF-2α (hypoxia-inducible factor 2α) to be compared with a nanomolar concentration of RSV or malvidin in proximal tubular cells of human kidneys (PTEC). Survival signaling systems activation (extracellular signal-regulated kinases, ERK and AMP-activated protein kinase, AMPK) was also investigated in PTEC incubated with wines. PTEC cells were incubated in the presence of RSV-poor wines diluted 1:1,000 for 30', 90', 120' and 24 h. Expression of SIRT1 and HIF-2α, and activation of ERK and AMPK were analyzed by Western Blot. The data obtained show that wine modulates the expression of anti-aging molecular systems even when RSV is present in very small amounts.
红酒酚类，特别是白藜芦醇（RSV）具有心脏保护和抗衰老作用，这是众所周知的。RSV 和其他天然存在的酚类物质最有趣的生物学特性之一是调节 SIRT1（沉默交配型信息调节 2 同源物）的表达和活性。鉴于 SIRT1 在急性和慢性肾脏疾病中的作用，我们决定研究 RSV 含量低的红酒对 SIRT1 和 HIF-2α（缺氧诱导因子 2α）表达的影响，并将其与纳摩尔浓度 RSV 或矢车菊素进行比较在人肾近端管状细胞（PTEC）中。还研究了葡萄酒孵育的 PTEC 中存活信号系统的激活（细胞外信号调节激酶，ERK 和 AMP 激活蛋白激酶，AMPK）。将 PTEC 细胞在稀释 1：1000 的 RSV 含量低的葡萄酒中孵育 30'、90'、120' 和 24 h。通过 Western Blot 分析 SIRT1 和 HIF-2α的表达以及 ERK 和 AMPK 的激活。获得的数据表明，即使 RSV 含量非常低，葡萄酒也能调节抗衰老分子系统的表达。
