Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, 30161-970 Belo Horizonte, MG, Brazil.
Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, 30161-970 Belo Horizonte, MG, Brazil.
Exp Gerontol. 2021 Apr;146:111226. doi: 10.1016/j.exger.2021.111226. Epub 2021 Jan 11.
One of the causes for aging is free radical damage. Resveratrol (RSV), a polyphenolic compound has been shown to act as an antioxidant and anti-inflammatory. The objective this study was to verify in an oxidative stress environment in Human Mononuclear cells from Middle aged and Elderly donors, the existence of a change in the SIRT1 and AMPK signaling pattern by RSV. In both age groups there was a reduction in reactive oxygen species (ROS) in cells stimulated with RSV. It was observed that in the Elderly group there was a higher production of ROS and that the reduction from RSV was smaller compared to the other group. There was an increased activity of Superoxide Dismutase in cells exposed to RSV in the elderly group. It was observed that for the Middle Aged group, SIRT 1 and AMPK are antioxidant pathways and RSV acts via SIRT1. In the elderly, the SIRT1 remains antioxidant and RSV ceases its operation via SIRT1. RSV has an antioxidant action in both age groups, and that in aging there was a change in the cellular context characterized by the silencing of the AMPK pathway antioxidant character.
衰老的原因之一是自由基损伤。白藜芦醇（RSV）是一种多酚化合物，已被证明具有抗氧化和抗炎作用。本研究的目的是在中年和老年供体的人单核细胞的氧化应激环境中，验证 RSV 是否改变 SIRT1 和 AMPK 信号通路。在两个年龄组中，用 RSV 刺激的细胞中活性氧（ROS）减少。观察到老年组中 ROS 的产生更高，并且与另一组相比，来自 RSV 的减少更小。在暴露于 RSV 的老年组细胞中超氧化物歧化酶的活性增加。观察到对于中年组，SIRT1 和 AMPK 是抗氧化途径，RSV 通过 SIRT1 起作用。在老年人中，SIRT1 仍然具有抗氧化作用，并且 RSV 通过 SIRT1 停止其作用。RSV 在两个年龄组均具有抗氧化作用，并且在衰老过程中，细胞环境发生变化，特征是 AMPK 途径抗氧化特性的沉默。
