白藜芦醇可上调人内脏脂肪细胞中的 SIRT1、FOXO1 和脂联素，并下调 PPARγ1-3 mRNA 的表达。
Resveratrol upregulated SIRT1, FOXO1, and adiponectin and downregulated PPARγ1-3 mRNA expression in human visceral adipocytes.
机构信息
Departamento de Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos 2600 Anexo, 90035-003, Porto Alegre, RS, Brazil.
出版信息
Obes Surg. 2011 Mar;21(3):356-61. doi: 10.1007/s11695-010-0251-7.
BACKGROUND
The SIRT1 enzyme is involved in adipose tissue (AT) lipolysis. FOXO1 is a protein that plays a significant role in regulating metabolism. Adiponectin is an adipokine, secreted by the AT, which has been considered to have an antiobesity function. PPARγ is one of the key actors in adipocytes differentiation. This study was undertaken to investigate whether resveratrol can regulate SIRT1, FOXO1, adiponectin, PPARγ1-3, and PPARβ/δ in human AT.
METHODS
The effects of resveratrol were analyzed in freshly isolated adipocytes prepared from visceral fat tissue samples obtained during bariatric surgery. Genes messenger ribonucleic acid (mRNA) levels were determined by qRT-PCR.
RESULTS
Ours results show that resveratrol modulates the studied genes, increasing SIRT1 (p = 0.021), FOXO1 (p = 0.001), and adiponectin (p = 0.025) mRNA expression and decreasing PPARγ1-3 (p = 0.003) mRNA in human visceral adipocytes.
CONCLUSIONS
Resveratrol, in vitro and at low concentration, modulates genes that are related to lipid metabolism, possibly preventing metabolic disease in human visceral adipose tissue (VAT).
背景
SIRT1 酶参与脂肪组织（AT）的脂解。FOXO1 是一种在调节代谢中起重要作用的蛋白质。脂联素是一种由 AT 分泌的脂肪因子，被认为具有抗肥胖作用。PPARγ 是脂肪细胞分化的关键因素之一。本研究旨在探讨白藜芦醇是否可以调节人 AT 中的 SIRT1、FOXO1、脂联素、PPARγ1-3 和 PPARβ/δ。
方法
分析了取自肥胖症手术期间获得的内脏脂肪组织样本的新鲜分离的脂肪细胞中白藜芦醇的作用。通过 qRT-PCR 测定基因信使核糖核酸（mRNA）水平。
结果
我们的结果表明，白藜芦醇调节研究的基因，增加 SIRT1（p=0.021）、FOXO1（p=0.001）和脂联素（p=0.025）mRNA 表达，并降低人内脏脂肪细胞中的 PPARγ1-3（p=0.003）mRNA。
结论
白藜芦醇在体外和低浓度下调节与脂质代谢相关的基因，可能预防人类内脏脂肪组织（VAT）中的代谢疾病。
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