Wu D, Xu W, Jiao R, Ding M, Zhai Z
Department of Biology, Peking University, Beijing.
Wei Sheng Wu Xue Bao. 1990 Dec;30(6):417-21.
The relationship of Sindbis virus (Sbv) assembly with intermediate filaments was studied by means of whole-mount and DGD embedment-free technique of EM together with the procedure of gentle extraction. In the early stage of Sbv infection, the "virus assembly center" was suspended in the intermediate filament network. In the late stage, the assembling and assembled virus nucleocapsids were associated with intermediate filaments. It is presumable that the virus nucleocapsids may move from the "virus assembly center" to cytoplasmic membrane along the intermediate filaments. Further study using immunolabelling technique indicated that the nonstructural protein 6K of Sbv was probably associated with intermediate filaments as well.
通过电子显微镜的整装和无DGD包埋技术以及温和提取程序，研究了辛德毕斯病毒（Sbv）组装与中间丝的关系。在Sbv感染的早期阶段，“病毒组装中心”悬浮在中间丝网络中。在后期，正在组装和已组装的病毒核衣壳与中间丝相关联。据推测，病毒核衣壳可能沿着中间丝从“病毒组装中心”移动到细胞质膜。使用免疫标记技术的进一步研究表明，Sbv的非结构蛋白6K可能也与中间丝相关。
