Wagner M, Veckenstedt A, Wagner B
Zentralbl Bakteriol Orig A. 1977 Apr;237(4):444-52.
Replication of Sindbis virus chick embryo fibroblasts was studied by means of the immunoferritin technique. Virus specific antigens, diffusely distributed in the cytoplasm, were observed at first 3 hours p.i. At the same time in ohter cells ferritin particles in linear arrangement occured at the surface of vesicles. At these places heavily labelled nucleocapsids were observed later on. This means, that the surface of vesicles is a site of assembly of nucleocapsids. Maturation of virus takes place at the cell surface as well as at the vesicles by budding. In early stages of replication maturation at the membrane prevalis.
采用免疫铁蛋白技术研究了辛德毕斯病毒在鸡胚成纤维细胞中的复制情况。接种病毒后3小时，首先观察到病毒特异性抗原，其在细胞质中呈弥漫性分布。与此同时，在其他细胞中，在线性排列的囊泡表面出现了铁蛋白颗粒。稍后在这些部位观察到了高度标记的核衣壳。这意味着，囊泡表面是核衣壳装配的场所。病毒通过出芽在细胞表面以及囊泡处成熟。在复制的早期阶段，在细胞膜处的成熟占优势。
