Maślanka T, Jaroszewski J J, Markiewicz W, Ziółkowski H, Barski D
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowski Street 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2012;15(1):11-20. doi: 10.2478/v10181-011-0108-6.
WC1+ cells in cattle exhibit both regulatory and effector activities. However, it has not been elucidated whether they are so plastic that both activities co-exist in one cell or there are separate subpopulations of effector and regulatory cells. Since the production of IFN-gamma and IL-10 seems to be related to WC1+ cells' effector and regulatory function, respectively, the main aim of this study was to determine whether those cytokines are produced by separate subpopulations of WC1+, or are co-produced by the same cells. Due to increasingly frequent emphasised role of consumption of IL-2 in the mechanism of suppressor action of mouse CD25+CD4+ T regulatory cells, expression of the receptor's alpha chain for interleukin 2 (CD25) on WC1+ lymphocytes has been evaluated. An average of 5.21% of WC1+ cells obtained from PBMCs of 12-month-old heifers show constitutive expression of the CD25 molecule, with CD25(high)WC1+ and CD25(low)WC1+ cells accounting for 1.05% and 4.10% of WC1+ lymphocytes, respectively. For detection of intracellular cytokine production, PBMCs were stimulated with concanavalin A. Both IFN-gamma(-) and IL-10-producing cells within the CD25(-)WC1+ and CD25+WC1+ subpopulations were mainly separate subpopulations. The average percentage of IFN-gamma(+)IL-10(-), IFN-gamma(-)IL-10+ and IFN-gamma(+)IL-10+ cells among CD25(-)WC1+ lymphocytes was 4.03%, 2.67% and 0.51%, respectively. A positive correlation was observed between the presence of the CD25 molecule on WC1+ lymphocytes and production of IL-10 and TGF-beta, because the average percentage of IFN-gamma(-)IL-10+ and IFN-gamma(+)IL-10+ among CD25+WC1+ lymphocytes was 3 and 4.5 times higher as compared to the corresponding cells in the CD25(-)WC1+ subpopulation, whereas the percentage of IFN-gamma(+)IL-10(-) cells in both the subpopulations was not significantly different. The percentage of TGF-beta+ cells within the CD25+WC1+ subpopulation was 2.72 times as high as that of CD25(-)WC1+ lymphocytes. Therefore, with respect to the production of IFN-gamma, IL-10 and TGF-beta, CD25+WC1+ lymphocytes turn out to have a more suppressor profile than CD25(-)WC1+.
牛体内的WC1+细胞兼具调节和效应活性。然而，它们是否具有如此的可塑性，以至于两种活性共存于一个细胞中，或者是否存在效应细胞和调节细胞的单独亚群，目前尚未阐明。由于干扰素-γ（IFN-γ）和白细胞介素-10（IL-10）的产生似乎分别与WC1+细胞的效应和调节功能相关，本研究的主要目的是确定这些细胞因子是由WC1+细胞的不同亚群产生，还是由同一细胞共同产生。由于白细胞介素-2（IL-2）消耗在小鼠CD25+CD4+ T调节细胞抑制作用机制中的作用日益受到重视，因此对WC1+淋巴细胞上白细胞介素2受体α链（CD25）的表达进行了评估。从12月龄小母牛外周血单核细胞（PBMC）中获得的WC1+细胞平均有5.21%显示CD25分子的组成性表达，其中CD25(高)WC1+和CD25(低)WC1+细胞分别占WC1+淋巴细胞的1.05%和4.10%。为检测细胞内细胞因子的产生，用刀豆蛋白A刺激PBMC。CD25(-)WC1+和CD25+WC1+亚群中产生IFN-γ(-)和IL-10的细胞主要是不同的亚群。CD25(-)WC1+淋巴细胞中IFN-γ(+)IL-10(-)、IFN-γ(-)IL-10+和IFN-γ(+)IL-10+细胞的平均百分比分别为4.03%、2.67%和0.51%。观察到WC1+淋巴细胞上CD25分子的存在与IL-10和转化生长因子-β（TGF-β）的产生之间存在正相关，因为CD25+WC1+淋巴细胞中IFN-γ(-)IL-10+和IFN-γ(+)IL-10+的平均百分比分别比CD25(-)WC1+亚群中相应细胞高3倍和4.5倍，而两个亚群中IFN-γ(+)IL-10(-)细胞的百分比无显著差异。CD25+WC1+亚群中TGF-β+细胞的百分比是CD25(-)WC1+淋巴细胞的2.72倍。因此，就IFN-γ、IL-10和TGF-β的产生而言，CD25+WC1+淋巴细胞比CD25(-)WC1+具有更强的抑制特征。
