Baquero Monica M, Plattner Brandon L
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Pathobiology/AHL Building 89, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Pathobiology/AHL Building 89, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
Cell Immunol. 2017 May;315:34-44. doi: 10.1016/j.cellimm.2017.01.009. Epub 2017 Jan 16.
The importance of bovine γδ T lymphocytes during anti-mycobacterial immunity is recognized; however, the role of major subsets of γδ T lymphocytes (WC1 and WC1) in this process remains unclear. We investigated how WC1 and WC1 γδ T lymphocyte subsets of calves modulate monocyte-derived macrophage (MDM) functions during Map infection in vitro. To achieve this, Map-infected or uninfected MDMs from young calves were co-cultured with autologous WC1 or WC1 γδ T lymphocytes. Our data indicate that WC1 and WC1 γδ T lymphocytes of young calves modulate effector functions of MDMs with respect to Map killing, CD11b and MHC-II expression. We observed differences in IFN-γ production and CD25 expression on γδ T lymphocyte subsets, as well as MDM expression of CD1b when in contact with WC1 γδ T lymphocytes.
牛γδ T淋巴细胞在抗分枝杆菌免疫过程中的重要性已得到认可；然而，γδ T淋巴细胞主要亚群（WC1和WC1）在此过程中的作用仍不清楚。我们研究了犊牛的WC1和WC1 γδ T淋巴细胞亚群在体外感染副结核分枝杆菌（Map）过程中如何调节单核细胞衍生巨噬细胞（MDM）的功能。为实现这一目标，将来自幼犊的感染或未感染Map的MDM与自体WC1或WC1 γδ T淋巴细胞共培养。我们的数据表明，幼犊的WC1和WC1 γδ T淋巴细胞在Map杀伤、CD11b和MHC-II表达方面调节MDM的效应功能。我们观察到γδ T淋巴细胞亚群的IFN-γ产生和CD25表达存在差异，以及与WC1 γδ T淋巴细胞接触时MDM的CD1b表达也存在差异。
