Zhang Z, Li Y, Zhang L L, Jia L T, Yang X Q
Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Tissue Antigens. 2012 Aug;80(2):158-64. doi: 10.1111/j.1399-0039.2012.01907.x. Epub 2012 Jun 18.
Preeclampsia (PE), especially severe PE including early (before 34 weeks' gestation) and late (after 34 weeks' gestation) onset PE, is one of the leading causes of maternal and fetal mortality and morbidity. It is well known that abnormal human leukocyte antigen subtype G (HLA-G) expression may contribute to PE. In this study, we investigated allelic and genotypic frequencies of the 14 bp deletion/insert polymorphism in the 3(')-untranslated region (3(')-UTR) of the HLA-G gene in cases (120 pairs of mother-offspring, 82 couples, and 67 pairs of father-offspring with severe PE) and controls (158 pairs of mother-offspring, 87 couples, and 75 pairs of father-offspring with normal pregnancy). We found that the frequencies of the +14 bp/+14 bp HLA-G genotype of the offspring were significantly higher in the severe and early onset severe PE cases compared with controls, and the frequencies of the -14 bp/-14 bp HLA-G genotype of the offspring were significantly lower in the early onset severe PE cases compared with controls. The frequency of combined -14 bp/+14 bp mother/+14 bp/+14 bp offspring genotypes was significantly higher in the severe and early onset severe PE cases compared with controls, and the frequency of combined -14 bp/+14 bp mother/-14 bp/-14 bp offspring genotypes was significantly lower in the early onset severe PE cases compared with late onset severe PE cases. The frequency of combined -14 bp/-14 bp father/-14 bp/-14 bp offspring genotypes was significantly lower in the early onset severe PE cases compared with late onset severe PE cases and controls. In overview, the HLA-G 14 bp deletion/insert polymorphism is associated with severe PE in father-offspring, and its distribution is different between the early and late onset severe PE.
子痫前期（PE），尤其是重度PE，包括早发型（妊娠34周前）和晚发型（妊娠34周后）PE，是孕产妇和围产儿发病和死亡的主要原因之一。众所周知，人类白细胞抗原G亚型（HLA-G）表达异常可能与PE有关。在本研究中，我们调查了HLA-G基因3′非翻译区（3′-UTR）14bp缺失/插入多态性的等位基因和基因型频率，研究对象包括病例组（120对母婴、82对夫妇以及67对父婴，均患有重度PE）和对照组（158对母婴、87对夫妇以及75对父婴，均为正常妊娠）。我们发现，与对照组相比，重度和早发型重度PE病例组中，子代+14bp/+14bp HLA-G基因型频率显著更高；与对照组相比，早发型重度PE病例组中，子代-14bp/-14bp HLA-G基因型频率显著更低。与对照组相比，重度和早发型重度PE病例组中，-14bp/+14bp母亲/+14bp/+14bp子代联合基因型频率显著更高；与晚发型重度PE病例组相比，早发型重度PE病例组中，-14bp/+14bp母亲/-14bp/-14bp子代联合基因型频率显著更低。与晚发型重度PE病例组和对照组相比，早发型重度PE病例组中，-14bp/-14bp父亲/-14bp/-14bp子代联合基因型频率显著更低。总体而言，HLA-G 14bp缺失/插入多态性与父婴重度PE有关，且在早发型和晚发型重度PE中的分布有所不同。
