Chrastil J
US Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70179.
Analyst. 1990 Oct;115(10):1383-4. doi: 10.1039/an9901501383.
A spectrophotometric method for the determination of cysteine and cystine in urine is described. This method is a modification of that reported for the determination of cysteine and cystine in proteins. The determination in urine is specific and simple. The colour develops at room temperature and no pre-treatment of urine is needed. Other amino acids, urea, uric acid, ascorbic acid, bilirubin, biliverdin, carbohydrates, hormones, acetone, salicylic acid, small amounts of protein and other common components of urine do not interfere. The method is particularly suitable for the diagnosis of hepatic cystinuria and other diseases characterised by high sulphur-containing amino acids in urine.
本文描述了一种用于测定尿液中半胱氨酸和胱氨酸的分光光度法。该方法是对已报道的用于测定蛋白质中半胱氨酸和胱氨酸方法的改进。尿液测定具有特异性且操作简单。在室温下显色，无需对尿液进行预处理。其他氨基酸、尿素、尿酸、抗坏血酸、胆红素、胆绿素、碳水化合物、激素、丙酮、水杨酸、少量蛋白质及尿液中的其他常见成分均不产生干扰。该方法特别适用于肝性胱氨酸尿症及其他以尿液中高含硫氨基酸为特征的疾病的诊断。
