College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Korea.
Microbiol Immunol. 2012 Sep;56(9):613-20. doi: 10.1111/j.1348-0421.2012.00486.x.
The aim of this study was to evaluate the immunomodulatory properties of Enterococcus faecium JWS 833 (JWS 833) isolated from duck intestine and compare them to those of Lactobacillus rhamnosus GG (LGG), a proven immunity-enhancing probiotic. To investigate the immune-enhancing properties of JWS 833, production of nitric oxide (NO) and cytokines was measured in mouse peritoneal macrophages. In addition, a Listeria monocytogenes challenge model was used in the assessment. It was found that heat-killed JWS 833 stimulates mouse peritoneal macrophages to produce NO, interleukin-1 β (IL-1β) and tumor necrosis factor-α (TNF-α) and that oral administration of viable JWS833 enhances NO, IL-1β and TNF-α synthesis upon L. monocytogenes challenge. Moreover, mice fed with JWS 833 were partially protected against lethal challenge with L. monocytogenes. JWS 833 strain has significantly greater immunostimulatory properties than LGG. Moreover, JWS 833 strain partially protects mice against lethal challenge with L. monocytogenes. JWS 833, a novel strain of E. faecium isolated from duck intestine, is potentially a useful feed supplement for controlling pathogens and enhancing host immune responses.
本研究旨在评估从鸭肠中分离出的屎肠球菌 JWS 833（JWS 833）的免疫调节特性，并将其与已被证实具有增强免疫功能的益生菌鼠李糖乳杆菌 GG（LGG）进行比较。为了研究 JWS 833 的免疫增强特性，测量了其对小鼠腹腔巨噬细胞一氧化氮（NO）和细胞因子的产生。此外，还使用李斯特菌单核细胞增生症（Listeria monocytogenes）挑战模型进行了评估。结果发现，热灭活的 JWS 833 可刺激小鼠腹腔巨噬细胞产生 NO、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α），而口服活的 JWS833 可增强李斯特菌单核细胞增生症（Listeria monocytogenes）挑战时的 NO、IL-1β 和 TNF-α 合成。此外，喂食 JWS 833 的小鼠对李斯特菌单核细胞增生症（Listeria monocytogenes）的致命性挑战有一定程度的保护作用。JWS 833 株具有比 LGG 更强的免疫刺激特性。此外，JWS 833 株可部分保护小鼠免受李斯特菌单核细胞增生症（Listeria monocytogenes）的致命性挑战。JWS 833 是一种从鸭肠中分离出的新型屎肠球菌，可能是一种有用的饲料添加剂，可用于控制病原体并增强宿主的免疫反应。
