Sashinami Hiroshi, Hu Dong-Liang, Li Sheng-Jun, Mitsui Toshihito, Hakamada Ken-Ichi, Ishiguro Yoh, Fukuda Shinsaku, Nakane Akio
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
FEMS Immunol Med Microbiol. 2010 Jun 1;59(1):100-7. doi: 10.1111/j.1574-695X.2010.00666.x. Epub 2010 Mar 4.
We investigated the effect of p60, a virulence factor of Listeria monocytogenes, on host immune response in vitro and in vivo. Administration of p60 before a sublethal infection with L. monocytogenes enhanced innate host resistance in naïve mice. Mouse macrophage RAW264.7 cells produced tumor necrosis factor (TNF)-alpha in response to stimulation with recombinant p60. Toll-like receptor 4 may be involved in TNF-alpha production from RAW264.7 cells and enhanced host resistance induced by p60 administration. Our findings demonstrated that p60 modulates innate immune responses against L. monocytogenes infection.
我们研究了单核细胞增生李斯特菌的毒力因子p60在体外和体内对宿主免疫反应的影响。在用单核细胞增生李斯特菌进行亚致死感染之前给予p60，可增强未接触过该菌的小鼠的先天宿主抵抗力。小鼠巨噬细胞RAW264.7细胞在受到重组p60刺激后会产生肿瘤坏死因子（TNF）-α。Toll样受体4可能参与RAW264.7细胞产生TNF-α以及p60给药诱导的宿主抵抗力增强。我们的研究结果表明，p60可调节针对单核细胞增生李斯特菌感染的先天免疫反应。
