Nathan Kline Institute for Psychiatric Research, Orangeburg, NY, USA.
J Neurosci Methods. 2012 Jul 30;209(1):195-8. doi: 10.1016/j.jneumeth.2012.06.005. Epub 2012 Jun 16.
A method is described for simultaneous histological processing of multiple fixed tissue samples. The tissue samples are embedded in a gelatin-albumin protein matrix that is rapidly solidified and bound to the tissue surface using a cross-linking reagent. After freezing and sectioning, the individual sections containing multiple samples can be processed for immunocytochemical and histochemical staining. The method is demonstrated for simultaneous processing of multiple rodent brains, and for reconstruction of fragmented human postmortem brain samples.
介绍了一种同时处理多个固定组织样本的组织学处理方法。将组织样本嵌入明胶-白蛋白蛋白基质中，该基质使用交联试剂迅速凝固并结合到组织表面。冷冻和切片后，可以对包含多个样本的单个切片进行免疫细胞化学和组织化学染色。该方法已成功用于同时处理多个啮齿动物大脑，以及重建碎片化的人类死后大脑样本。
