Griffioen H A, Van der Beek E, Boer G J
Netherlands Institute for Brain Research, Amsterdam.
J Neurosci Methods. 1992 Jun;43(1):43-7. doi: 10.1016/0165-0270(92)90065-l.
Vibratome sectioning of paraformaldehyde-fixed brains containing a lesion and/or intracerebroventricular grafts and staining of these sections often lead to damage around the site of the lesion and loss of the implants. Various embedding procedures were compared in order to find a method to overcome this problem. The best results were obtained when fixed brain tissue was first embedded under reduced pressure in 10% gelatin at 50 degrees C, and then fixed again, in 4% paraformaldehyde. The method did not affect the immunocytochemical staining for vasopressin, vasoactive intestinal polypeptide, somatostatin, gonadotropin releasing hormone and neuropeptide Y.
对含有损伤和/或脑室内移植体的多聚甲醛固定脑进行振动切片以及对这些切片进行染色，常常会导致损伤部位周围的损伤以及植入物的丢失。为了找到一种克服此问题的方法，对各种包埋程序进行了比较。当将固定的脑组织首先在减压条件下于50摄氏度嵌入10%的明胶中，然后再用4%的多聚甲醛固定时，可获得最佳结果。该方法不影响抗利尿激素、血管活性肠多肽、生长抑素、促性腺激素释放激素和神经肽Y的免疫细胞化学染色。
