Department of Fetal Medicine, 4002, Rigshospitalet Copenhagen University Hospital, Copenhagen, Denmark.
Prenat Diagn. 2012 Sep;32(9):840-5. doi: 10.1002/pd.3917. Epub 2012 Jun 18.
To assess whether spontaneous preterm delivery can be predicted from the amount of cell free fetal DNA (cffDNA) as determined by routine fetal RHD genotyping at 25 weeks' gestation.
Cohort study including RhD negative women participating in a routine RHD screening programme. A standard dilution curve was used to quantify the amounts of cffDNA. Values above the 95(th) centile for the study population defined high levels of cffDNA.
We found a highly significant association between preterm delivery and cffDNA levels above the 95(th) centile (p = 0.002). Using logistic regression, women with high levels of cffDNA had an odds ratio of 6.3 (95% confidence interval: 1.9-20.9) for preterm delivery before 37 weeks and an odds ratio for delivery before 34 weeks of 16.6 (95% confidence interval: 3.2-84.7) when adjusting for gestational age at sampling, body mass index and previous miscarriages/terminations of pregnancy.
High levels of cffDNA at 25 weeks are associated with increased risk of spontaneous preterm delivery.
评估在 25 周进行常规胎儿 RHD 基因分型时，通过游离胎儿 DNA（cffDNA）的量是否可以预测自发性早产。
纳入 RhD 阴性女性的队列研究，她们参加常规的 RHD 筛查计划。使用标准稀释曲线来定量 cffDNA 的量。研究人群中 95 百分位以上的数值定义为 cffDNA 高水平。
我们发现早产与 95 百分位以上的 cffDNA 水平之间存在高度显著的关联（p=0.002）。使用逻辑回归，cffDNA 水平较高的女性在 37 周前早产的优势比为 6.3（95%置信区间：1.9-20.9），调整采样时的孕龄、体重指数和既往流产/终止妊娠后，34 周前分娩的优势比为 16.6（95%置信区间：3.2-84.7）。
25 周时 cffDNA 水平较高与自发性早产风险增加相关。
