Martinello Tiziana, Bronzini Ilaria, Volpin Andrea, Vindigni Vincenzo, Maccatrozzo Lisa, Caporale Giovanni, Bassetto Franco, Patruno Marco
University of Padova, Department of Comparative Biomedicine and Food Science, Italy.
J Tissue Eng Regen Med. 2014 Aug;8(8):612-9. doi: 10.1002/term.1557. Epub 2012 Jun 19.
The major goal of regenerative medicine is to determine experimental techniques that take maximal advantage of reparative processes that occur naturally in the animal body. Injection of mesenchymal stem cells into the core of a damaged tendon represents such an approach. Decellularization of native tendons as potential targets and seeding protocols are currently under investigation. The aim of our study was to manufacture a recellularized biocompatible scaffold from cadaveric tissue for use in total or partial tendon injuries. Results showed that it was possible to introduce proliferating cells into the core of a decellularized tendon to treat the scaffold with a collagen gel. The method was effective in maintaining scaffold extracellular matrix and for expressing collagen type I and cartilage oligomeric matrix protein by injecting mesenchymal stem cells.
再生医学的主要目标是确定能最大程度利用动物体内自然发生的修复过程的实验技术。将间充质干细胞注射到受损肌腱的核心就是这样一种方法。目前正在研究将天然肌腱脱细胞作为潜在靶点以及接种方案。我们研究的目的是用尸体组织制造一种重新细胞化的生物相容性支架，用于治疗全部或部分肌腱损伤。结果表明，将增殖细胞引入脱细胞肌腱的核心并用胶原凝胶处理支架是可行的。该方法通过注射间充质干细胞，在维持支架细胞外基质以及表达I型胶原蛋白和软骨寡聚基质蛋白方面是有效的。
