De Nguyen Van, Le Thanh Hoa, Chai Jong-Yil
Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam.
Korean J Parasitol. 2012 Jun;50(2):137-41. doi: 10.3347/kjp.2012.50.2.137. Epub 2012 May 24.
From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.
2006年至2010年期间，河内的医院收治了10名盘尾丝虫病患者。从寄生部位采集虫体，并通过形态学和分子方法完成虫种鉴定。10条寄生虫分别从结膜（n = 9）或皮下组织（n = 1）中获取。这些寄生虫体长4.0 - 12.5厘米，宽0.5 - 0.6毫米。形态学观察表明所有寄生虫均为匐行恶丝虫。10条寄生虫中的3条（1条来自皮下组织，2条来自眼部）用于虫种鉴定的分子确认。扩增并测序了线粒体细胞色素氧化酶亚基1（cox1）的一部分（461 bp）。与匐行恶丝虫（意大利来源，GenBank AJ271614；DQ358814）相比，核苷酸和氨基酸同源性分别为95%和99 - 100%。这是越南首例关于眼部盘尾丝虫病的报告，也是第二例由匐行恶丝虫引起的皮下组织盘尾丝虫病报告。
