Ghedin Elodie, Wang Shiliang, Spiro David, Caler Elisabet, Zhao Qi, Crabtree Jonathan, Allen Jonathan E, Delcher Arthur L, Guiliano David B, Miranda-Saavedra Diego, Angiuoli Samuel V, Creasy Todd, Amedeo Paolo, Haas Brian, El-Sayed Najib M, Wortman Jennifer R, Feldblyum Tamara, Tallon Luke, Schatz Michael, Shumway Martin, Koo Hean, Salzberg Steven L, Schobel Seth, Pertea Mihaela, Pop Mihai, White Owen, Barton Geoffrey J, Carlow Clotilde K S, Crawford Michael J, Daub Jennifer, Dimmic Matthew W, Estes Chris F, Foster Jeremy M, Ganatra Mehul, Gregory William F, Johnson Nicholas M, Jin Jinming, Komuniecki Richard, Korf Ian, Kumar Sanjay, Laney Sandra, Li Ben-Wen, Li Wen, Lindblom Tim H, Lustigman Sara, Ma Dong, Maina Claude V, Martin David M A, McCarter James P, McReynolds Larry, Mitreva Makedonka, Nutman Thomas B, Parkinson John, Peregrín-Alvarez José M, Poole Catherine, Ren Qinghu, Saunders Lori, Sluder Ann E, Smith Katherine, Stanke Mario, Unnasch Thomas R, Ware Jenna, Wei Aguan D, Weil Gary, Williams Deryck J, Zhang Yinhua, Williams Steven A, Fraser-Liggett Claire, Slatko Barton, Blaxter Mark L, Scott Alan L
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
Science. 2007 Sep 21;317(5845):1756-60. doi: 10.1126/science.1145406.
Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.
导致象皮病和河盲症的寄生线虫威胁着发展中国家数亿人的健康。我们已对人类丝虫寄生虫马来布鲁线虫（Brugia malayi）约90兆碱基（Mb）的基因组进行了测序，并在71 Mb的可靠组装序列中预测了约11,500个蛋白质编码基因。与自由生活的模式线虫秀丽隐杆线虫（Caenorhabditis elegans）进行的比较分析表明，尽管这些基因在约3.5亿年的进化过程中局部共线性的保守性很低，但它们在染色体单元上基本仍保持连锁状态。已鉴定出100多个保守操纵子。对预测蛋白质组的分析为马来布鲁线虫适应其人类宿主和媒介宿主的生态位提供了证据，并深入了解了其与沃尔巴克氏体（Wolbachia）内共生菌互利共生关系的分子基础。这些发现为合理的药物设计奠定了基础。
