Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
Biotechnol Lett. 2012 Oct;34(10):1807-10. doi: 10.1007/s10529-012-0982-8. Epub 2012 Jun 20.
The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.
在摇瓶培养中研究了聚合物添加剂（包括一组粘度增强聚合物聚γ-谷氨酸（γPGA；10、50 和 500 kDa）和表面活性剂聚合物 Pluronic F68）对中国仓鼠卵巢细胞免受剪切力损伤的保护作用。保护水平取决于 γPGA 的分子量及其浓度。当添加 0.05 或 0.075%的 500 kDa γPGA 时，细胞生长和活力几乎与添加 Pluronic F68 时相同，比没有添加剂的对照高得多。我们首次表明，γPGA 是另一种环保型培养基添加剂，可以替代 Pluronic F68 使用。
