Wu J, Daugulis A J, Faulkner P, Goosen M F
Department of Chemical Engineering, Queen's University at Kingston, Ontario, Canada.
Biotechnol Prog. 1995 Mar-Apr;11(2):127-32. doi: 10.1021/bp00032a002.
The protective effects of polymer additives on insect cells against fluid mechanical damage was investigated in a falling film-flow device. The falling liquid film creates rapidly moving air-liquid interfaces and high fluid shear stress, mimicking the characteristics of a bursting bubble in aerated cell culture. The additives tested included a group of surface-active polymers, (i.e., Pluronic F68, poly(ethylene glycol)s, and Tween 80) and a group of viscosity-enhancing polymers (i.e., dextrans, methyl-cellulose, and (carboxymethyl)cellulose). We found that methylcellulose, which was previously considered a viscosity-enhancing polymer, actually had significant surface-active properties. All of the surface-active polymers exhibited significant protective effects, with Pluronic F68 and the higher molecular weight poly(ethylene glycol), PEG 20M, providing the best protection. In contrast, the viscosity-enhancing polymers, with the exception of methylcellulose, showed little or no protection for insect cells in the film flow. All of the protective polymers had surface-active properties, even though some of them did not change the surface tension in the actual insect cell medium. There was no correlation between the protective effect and the changes in liquid viscosity and surface tension due to the polymer additives. The level of protection was shown to be dependent upon the type of polymer, its concentration in the culture medium, and the polymer molecular weight. We concluded that the mechanism of protection of these surface-active polymers was through interaction of the polymer molecules with the cell plasma membranes: a fast-acting biological mechanism.
在降膜流动装置中研究了聚合物添加剂对昆虫细胞免受流体机械损伤的保护作用。下落的液膜会产生快速移动的气液界面和高流体剪切应力，模拟了充气细胞培养中破裂气泡的特征。测试的添加剂包括一组表面活性聚合物（即普朗尼克F68、聚乙二醇和吐温80）和一组增粘聚合物（即葡聚糖、甲基纤维素和羧甲基纤维素）。我们发现，甲基纤维素以前被认为是一种增粘聚合物，实际上具有显著的表面活性特性。所有表面活性聚合物都表现出显著的保护作用，其中普朗尼克F68和较高分子量的聚乙二醇PEG 20M提供了最佳保护。相比之下，除甲基纤维素外，增粘聚合物在膜流中对昆虫细胞几乎没有保护作用。所有保护聚合物都具有表面活性特性，尽管其中一些在实际昆虫细胞培养基中并未改变表面张力。聚合物添加剂引起的保护作用与液体粘度和表面张力的变化之间没有相关性。保护水平取决于聚合物的类型、其在培养基中的浓度以及聚合物分子量。我们得出结论，这些表面活性聚合物的保护机制是通过聚合物分子与细胞质膜的相互作用：一种快速起效的生物学机制。
