静脉麻醉剂对小鼠胚胎成纤维细胞活力和增殖的影响。
The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation.
机构信息
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan, Seoul, Korea.
出版信息
J Anesth. 2012 Oct;26(5):675-81. doi: 10.1007/s00540-012-1427-1. Epub 2012 Jun 20.
PURPOSE
The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system.
METHODS
The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit.
RESULTS
Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time.
CONCLUSION
Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.
目的
本研究旨在评估静脉麻醉剂对体外培养的小鼠成纤维细胞的细胞毒性和增殖抑制作用。
方法
将细胞暴露于静脉麻醉剂的常用临床血浆浓度下，即咪达唑仑（0.15μg/ml）、丙泊酚（2μg/ml）、瑞芬太尼（2μg/ml）、硫喷妥钠（10μg/ml），分别暴露 4、8 或 24 小时。使用 MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）测定法分析静脉麻醉剂下的细胞增殖（每组 6 个）。使用 LIVE/DEAD 活力测定试剂盒研究静脉麻醉剂的细胞毒性（每组 6 个）。
结果
静脉麻醉剂暴露时间不影响小鼠成纤维细胞的增殖率。静脉麻醉剂的细胞毒性与暴露时间无关。
结论
我们的结果表明，静脉麻醉剂可能不会影响小鼠成纤维细胞的增殖和活力。
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