Federal University of São Paulo, SP, Brazil.
Braz J Otorhinolaryngol. 2012 Jun;78(3):14-8. doi: 10.1590/S1808-86942012000300003.
The vibration of the vocal fold lamina propria is an important factor involved in vocal production and aging may change the amount of hyaluronic acid in the vocal fold leading to dysphonia.
This study compares the concentration of hyaluronic acid in vocal folds of aged and young female rats.
experimental.
We used the vocal cords of 13 female rats divided into two groups: five aged rats and eight young ones. The tissue concentration of hyaluronic acid was determined using the fluorimetric method with the hyaluronic acid binding-protein coated on plates of enzyme-linked immunosorbent assay (ELISA), conjugated with biotin. Europium-labeled streptavidin was added and, after europium release with the use of enhancement solution, the final fluorescence was measured in a fluorometer.
We found the following concentrations of hyaluronic acid in vocal fold according to the group: 581.7 ng/mg in old female rats and 1275.6 ng/mg in young female rats. Statistical analysis showed differences between groups.
The vocal folds of old female rats have a lower concentration of hyaluronic acid when compared to such concentration on the vocal folds of young female rats.
声带固有层的振动是参与发声的一个重要因素，而衰老可能会改变声带中的透明质酸含量，导致声音障碍。
本研究比较了老年和年轻雌性大鼠声带中透明质酸的浓度。
实验性。
我们使用了 13 只雌性大鼠的声带，分为两组：5 只老年大鼠和 8 只年轻大鼠。使用酶联免疫吸附试验（ELISA）中带有透明质酸结合蛋白的平板，通过荧光法测定组织中透明质酸的浓度，与生物素偶联。加入铕标记的链霉亲和素，并用增强溶液释放铕后，在荧光计中测量最终荧光。
根据组的不同，我们在声带中发现了以下浓度的透明质酸：老年雌性大鼠为 581.7ng/mg，年轻雌性大鼠为 1275.6ng/mg。统计学分析显示组间存在差异。
与年轻雌性大鼠声带中的浓度相比，老年雌性大鼠声带中的透明质酸浓度较低。
