Department of Pharmacology, University of Florence, Florence, Italy.
Mol Nutr Food Res. 2012 Aug;56(8):1353-7. doi: 10.1002/mnfr.201200122. Epub 2012 Jun 20.
Inflammation may increase cancer risk, therefore, we studied whether polyphenol-rich Marie Ménard (MM) apples with reported anti-inflammatory activity prevent 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats and, likewise whether high-fat (HF) diet promoting carcinogenesis, may affect inflammation. DMH-induced rats were fed for 15 weeks with: an HF diet (23% corn oil w/w); an HF diet containing 7.6% w/w lyophilized MM (apple diet (AD)); a low-fat (LF) diet and an HF diet containing piroxicam (PXC) (0.01% w/w) as control. Mucin depleted foci (MDF), precancerous lesions in the colon, were dramatically reduced in the AD, LF, and PXC groups compared with the HF. Peritoneal macrophage activation, an index of systemic inflammation, was significantly decreased in the AD, LF, and PXC groups. TNF-α, iNOS, IL-1β, IL-6 m-RNA expression in the colon, as well as CD68 cells and plasmatic PGE2 were lower in the AD, but not in the LF group. Apoptosis in the MDF of both the AD and LF-fed rats was significantly higher than in HF rats. In conclusion, AD has a strong chemopreventive effect, reducing inflammation, and increasing apoptosis, while the chemopreventive effect of the LF diet seems mediated mainly by increased apoptosis in MDF.
炎症可能会增加癌症风险，因此，我们研究了富含多酚的 Marie Ménard（MM）苹果是否具有抗炎活性，能否预防 1,2-二甲基肼（DMH）诱导的大鼠结肠癌发生，以及高脂肪（HF）饮食促进致癌作用是否会影响炎症。用以下饮食喂养 DMH 诱导的大鼠 15 周：含有 23%玉米油（w/w）的 HF 饮食；含有 7.6%冻干 MM（苹果饮食（AD））的 HF 饮食；低脂肪（LF）饮食和含有吡罗昔康（PXC）的 HF 饮食（作为对照，0.01% w/w）。与 HF 组相比，AD、LF 和 PXC 组的粘蛋白耗竭灶（MDF），即结肠的癌前病变明显减少。AD、LF 和 PXC 组的腹膜巨噬细胞活化，作为全身炎症的指标，明显降低。AD 组结肠 TNF-α、iNOS、IL-1β、IL-6 m-RNA 表达以及 CD68 细胞和血浆 PGE2 降低，但 LF 组没有。AD 和 LF 喂养大鼠的 MDF 中的细胞凋亡明显高于 HF 大鼠。总之，AD 具有很强的化学预防作用，可降低炎症并增加细胞凋亡，而 LF 饮食的化学预防作用似乎主要通过增加 MDF 中的细胞凋亡来介导。
