Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan, R.O.C.
Chem Asian J. 2012 Sep;7(9):2073-9. doi: 10.1002/asia.201200222. Epub 2012 Jun 19.
We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAb(H5)/SiNW-FET) after detecting approximately 10(-17) M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.
我们应用了一种可重复使用的硅纳米线场效应晶体管（SiNW-FET）作为生物传感器，对非常稀释溶液中的 H5N2 禽流感病毒（AIV）进行超灵敏检测。通过使用二硫键连接物，实现了 SiNW-FET 的可逆表面功能化。在表面功能化中，首先在 SiNW-FET 上修饰 3-巯基丙基三甲氧基硅烷（MPTMS）（称为 MPTMS/SiNW-FET），随后用二硫苏糖醇（DTT）洗涤以减少 MPTMS 中巯基之间可能形成的任何二硫键。随后，通过形成二硫键，可以将受体分子固定在 MPTMS/SiNW-FET 上。通过荧光检查和电学测量验证了可逆表面功能化的成功。用原子力显微镜扫描了用针对 H5N2 病毒的单克隆抗体（称为 mAb（H5）/SiNW-FET）修饰后的 SiNW-FET 生物传感器的表面形貌，以证明 SiNW-FET 能够检测到非常少量的 H5N2 AIV 颗粒。
