Wålinder O, Karlsson F A, Dahlberg P A
Acta Endocrinol (Copenh). 1979 Sep;92(1):95-104. doi: 10.1530/acta.0.0920095.
The adenylcyclase (AC) activity of crude human thyroid plasma membranes were studied in some detail and conditions for optimal cyclic AMP-production established. Membranes from eight "cold" and two "hot" thyroid adenomas were investigated and compared to membranes from corresponding normal, paranodular tissues. The investigated membranes were found to contain similar basal AC activities, which were stimulated three to five times with TSH and 20--30 times with fluoride. Further, no difference in the TSH-sensitivity of AC from normal and "cold" adenomas could be detected. Thyrotrophin 0.02 mU/ml caused a measurable and 0.15 mU/ml a half-maximal stimulation of the cAMP-production. The data indicate, that disturbances of thyroid hormone production characteristic of thyroid adenomas, are not caused by alterations of the TSH-AC system and suggest that in a thyroid cell the degree of thyroid hormone synthesis do not alter the amount of plasma membrane-bound adenylcyclase.
对人甲状腺粗制质膜的腺苷酸环化酶（AC）活性进行了较为详细的研究，并确定了产生最佳环磷酸腺苷（cAMP）的条件。研究了来自8个“冷”甲状腺腺瘤和2个“热”甲状腺腺瘤的质膜，并与相应正常、结节旁组织的质膜进行了比较。发现所研究的质膜具有相似的基础AC活性，TSH可使其活性提高3至5倍，氟化物可使其提高20至30倍。此外，未检测到正常和“冷”腺瘤的AC对TSH敏感性的差异。0.02 mU/ml的促甲状腺激素可引起可测量的cAMP产生刺激，0.15 mU/ml可引起半最大刺激。数据表明，甲状腺腺瘤特有的甲状腺激素产生紊乱并非由TSH-AC系统的改变引起，这表明在甲状腺细胞中，甲状腺激素合成的程度不会改变质膜结合腺苷酸环化酶的量。
