Department of Gynecology, Federal University of Piauí, Teresina, Piauí, Brazil.
Eur J Obstet Gynecol Reprod Biol. 2012 Sep;164(1):102-4. doi: 10.1016/j.ejogrb.2012.05.036. Epub 2012 Jun 19.
The aim was to evaluate the effects of tamoxifen in activating extracellular signal-regulated kinases (ERKs) 1 and 2 in the urethras of castrated female rats.
Twelve castrated adult female rats were divided into a control group (n=6) in which the animals received vehicle, and the experimental group (n=6) in which the rats received tamoxifen 250 μg/day by gavage for 28 days. Then, the animals were sacrificed and their urethras removed. Proteins were extracted, quantified and processed by Western blot analysis with specific phospho-ERK1 and 2 antibodies. Data were analyzed using Student's t-test (p<0.05).
A significant increase occurred in phospho-ERK1 levels in the experimental group compared to the control group (p<0.01), while no difference was found in phospho-ERK2 levels between the groups (p=0.313).
The present results indicate that, at the doses and during the time of treatment used, tamoxifen significantly increased phospho-ERK1 levels in the urethras of castrated female rats.
评估他莫昔芬激活去势雌性大鼠尿道细胞外信号调节激酶（ERK）1 和 2 的效果。
将 12 只去势成年雌性大鼠分为对照组（n=6）和实验组（n=6）。对照组大鼠给予载体，实验组大鼠给予他莫昔芬 250μg/天灌胃，共 28 天。然后处死大鼠并取出尿道。用特定的磷酸化 ERK1 和 2 抗体提取、定量和处理蛋白质。采用 Student's t 检验分析数据（p<0.05）。
与对照组相比，实验组磷酸化 ERK1 水平显著升高（p<0.01），而两组磷酸化 ERK2 水平无差异（p=0.313）。
本研究结果表明，在使用的剂量和治疗时间内，他莫昔芬显著增加了去势雌性大鼠尿道中磷酸化 ERK1 的水平。
