Department of Gynecology, Federal University of Piauí, Teresina, Piauí, Brazil.
Gynecol Endocrinol. 2009 May;25(5):282-6. doi: 10.1080/09513590802488420.
The aim of this study was to evaluate the effects of tamoxifen on vascular endothelial growth factor (VEGF) expression in the urethral epithelium of castrated rats.
Thirty-six adult, castrated, female Wistar-Hannover rats were randomly divided into two groups: group I (n = 16, control), receiving only propylene glycol, and group II (n = 20, tamoxifen), treated with 250 microg/day of tamoxifen for 30 consecutive days by gavage. On the 31st day, the animals were sacrificed and the urethras were immediately removed, separated into the proximal and distal segments and processed for VEGF immunohistochemistry. The data were analysed using Student's t-test (p < 0.05).
The mean percentage of VEGF expression in the epithelium of the proximal urethra of the animals in groups I and II was 64.47+/-3.70 and 74.69+/-3.03, respectively (p < 0.03), whereas the mean percentage of VEGF expression in the distal urethral epithelium of the animals in groups I and II was 53.49+/-4.64 and 68.57+/-3.67, respectively (p < 0.01).
Our results indicate that, at the dose and during the time of treatment used, tamoxifen increased VEGF expression in the urethral epithelium of castrated rats.
本研究旨在评估他莫昔芬对去势大鼠尿道上皮血管内皮生长因子（VEGF）表达的影响。
36 只成年雌性 Wistar-Hanover 去势大鼠随机分为两组：I 组（n = 16，对照组），仅给予丙二醇；II 组（n = 20，他莫昔芬组），通过灌胃给予 250μg/天他莫昔芬连续 30 天。第 31 天处死动物，立即取出尿道，分离为近段和远段，用于 VEGF 免疫组化。使用 Student's t 检验（p < 0.05）对数据进行分析。
I 组和 II 组大鼠近段尿道上皮 VEGF 表达的平均百分比分别为 64.47+/-3.70 和 74.69+/-3.03（p < 0.03），而 I 组和 II 组大鼠远段尿道上皮 VEGF 表达的平均百分比分别为 53.49+/-4.64 和 68.57+/-3.67（p < 0.01）。
我们的结果表明，在使用的剂量和治疗时间内，他莫昔芬增加了去势大鼠尿道上皮的 VEGF 表达。
