Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada P7B 5E1.
Ecotoxicol Environ Saf. 2012 Aug;82:80-4. doi: 10.1016/j.ecoenv.2012.05.011. Epub 2012 Jun 19.
In this study an electroantennogram (EAG) method was developed for use on live daphniids. The EAG response of Daphnia magna and Daphnia pulex to a variety of amino acids was measured. The strongest response measured was elicited by L-arginine and was shown to induce a concentration-dependent response indicating the response is olfactory in nature. Subsequent exposures of D. magna to a low, ecologically-relevant concentration of copper (7.5 μg/L) showed a disruption in EAG function. This study utilizes the development of an EAG method for measuring olfactory acuity of live daphniids and demonstrates that at ecologically-relevant concentrations, the olfactory dysfunction caused by copper can be detected. The EAG technique is a useful tool for investigating the olfactory response of daphniids to odourants at the cellular level and detecting the effects of toxicants on the olfactory acuity of daphniids.
在这项研究中，开发了一种用于活体水蚤的触角电位（EAG）方法。测量了大型溞和双齿多刺水蚤对各种氨基酸的 EAG 反应。测量到的最强反应是由 L-精氨酸引起的，并且表明该反应是嗅觉性质的，呈浓度依赖性。随后，将大型溞暴露于低浓度（7.5μg/L）的铜，这一浓度在生态上具有相关性，结果显示 EAG 功能受到干扰。本研究利用 EAG 方法测量活体水蚤的嗅觉敏锐度，并证明在生态相关浓度下，可以检测到铜引起的嗅觉功能障碍。EAG 技术是一种用于在细胞水平上研究水蚤对气味的嗅觉反应以及检测毒物对水蚤嗅觉敏锐度影响的有用工具。
