Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.
Res Vet Sci. 2012 Dec;93(3):1454-61. doi: 10.1016/j.rvsc.2012.05.009. Epub 2012 Jun 19.
Adapter-modified Ussing chambers have been used for assessment of endoscopically obtained intestinal biopsies in humans. The aim of this study was to evaluate the feasibility of an adapter-modified Ussing chamber for assessment of intestinal transport physiology in endoscopically-obtained colonic biopsies from cats and dogs. Fifteen colonic biopsies from four cats and 13 colonic biopsies from four dogs were transferred into a modified Ussing chamber and sequentially exposed to several compounds. Baseline mean±SD conductance was measured. Changes of short circuit current (ΔIsc) were observed after exposure to glucose (number of feline biopsies that responded=0/number of canine biopsies that responded=4), phloridzin (n=0/n=7), histamine (n=5/n=12), serotonin (n=7/n=12), prostaglandin (n=5/n=7), forskolin (n=7/n=7), and ouabain (n=9/n=7). The adapter-modified Ussing chamber studied here enables investigation of transport physiology of endoscopically-obtained colonic biopsies from companion animals. However, we observed a large variability of results, suggesting that clinical use of this method is limited.
适配器改良的 Ussing 室已被用于评估人类内窥镜获得的肠活检标本的功能。本研究旨在评估适配器改良的 Ussing 室用于评估猫和狗内窥镜获得的结肠活检标本肠转运生理学的可行性。将 15 个来自 4 只猫的结肠活检标本和 13 个来自 4 只狗的结肠活检标本转移到改良的 Ussing 室中，并依次暴露于几种化合物。测量基础（无干预）时的平均±SD 电导率。观察到暴露于葡萄糖（猫的活检标本中有 0/犬的活检标本中有 4 个有反应）、根皮苷（n=0/犬的活检标本中有 7 个有反应）、组胺（n=5/犬的活检标本中有 12 个有反应）、5-羟色胺（n=7/犬的活检标本中有 12 个有反应）、前列腺素（n=5/犬的活检标本中有 7 个有反应）、福司可林（n=7/犬的活检标本中有 7 个有反应）和哇巴因（n=9/犬的活检标本中有 7 个有反应）后的短路电流（ΔIsc）变化。本研究中使用的适配器改良 Ussing 室可用于研究伴侣动物内窥镜获得的结肠活检标本的转运生理学。然而，我们观察到结果的变异性很大，这表明该方法的临床应用受到限制。
