Ruhnke Isabelle, DeBiasio John V, Suchodolski Jan S, Newman Shelley, Musch Mark W, Steiner Jörg M
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474,
J Vet Sci. 2014;15(2):297-307. doi: 10.4142/jvs.2014.15.2.297. Epub 2013 Dec 27.
This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from cats responded to at least one compound. In duodenal biopsies obtained from dogs, the rate of overall response ranged from 87.5% (healthy individuals; n = 8), to 63.6% (animals exhibiting clinical signs of gastrointestinal disease and histopathological unremarkable duodenum; n = 15), and 32.1% (animals exhibiting clinical signs of gastrointestinal diseases and moderate to severe histopathological lesions; n = 28). Detailed information regarding the magnitude and duration of the response are provided. The adapter-modified Ussing chamber enables investigation of the absorptive and secretory capacity of endoscopically obtained duodenal biopsies from cats and dogs and has the potential to become a valuable research tool. The response of samples was correlated with histopathological findings.
本研究旨在评估一种经适配器改良的尤斯灌流小室，用于评估从健康猫和狗以及患有慢性肠病的狗的十二指肠内镜活检组织中的转运生理学。从5只猫身上获取了17份十二指肠活检组织，从13只狗身上获取了51份十二指肠活检组织。将样本转移至经适配器改良的尤斯灌流小室，并依次暴露于各种促吸收剂和促分泌剂。总体而言，从猫身上获取的十二指肠样本中，78.6%对至少一种化合物有反应。在从狗身上获取的十二指肠活检组织中，总体反应率从87.5%（健康个体；n = 8）到63.6%（表现出胃肠道疾病临床症状且十二指肠组织病理学无明显异常的动物；n = 15），以及32.1%（表现出胃肠道疾病临床症状且有中度至重度组织病理学病变的动物；n = 28）。提供了有关反应幅度和持续时间的详细信息。经适配器改良的尤斯灌流小室能够研究从猫和狗的十二指肠内镜活检组织中的吸收和分泌能力，并且有潜力成为一种有价值的研究工具。样本的反应与组织病理学结果相关。
