一种改良的、高灵敏度的高尔基-考克斯（Golgi-Cox）方法，可实现胚胎神经元的完全和稳定浸渍。
A modified and highly sensitive Golgi-Cox method to enable complete and stable impregnation of embryonic neurons.
机构信息
Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
出版信息
J Neurosci Methods. 2012 Jul 30;209(1):58-61. doi: 10.1016/j.jneumeth.2012.06.007. Epub 2012 Jun 18.
The FD Rapid GolgiStain Kit (FD NeuroTechnologies, Inc., Ellicott City, MD) was developed using an improved Golgi-Cox impregnation method to provide stable, sensitive, and convenient staining of mature neurons. However, embryonic neurons cannot be clearly visualized using this method. We have further improved the Golgi-Cox method by introducing an additional aldehyde fixation step before impregnation; thus, we can stably and sufficiently impregnate fetal brain neurons so that even the fine branches of the dendritic trees are visible.
FD 快速高尔基体染色试剂盒（FD 神经技术公司，马里兰州埃利科特市）采用改良的高尔基体-考克斯浸染法开发，为成熟神经元提供稳定、敏感和便捷的染色。然而，该方法不能清晰显示胚胎神经元。我们通过在浸染前引入额外的醛固定步骤进一步改进了高尔基体-考克斯方法；因此，我们可以稳定且充分地浸染胎脑神经元，使树突的精细分支也清晰可见。
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