有丝分裂过程中，着丝粒蛋白 B 的分离酶依赖性裂解对于中心体脱离是必要且充分的。

Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis.

机构信息

Department of Biological Sciences, Seoul National University, Korea.

出版信息

Cell Cycle. 2012 Jul 1;11(13):2476-85. doi: 10.4161/cc.20878.

DOI:10.4161/cc.20878

Abstract

Centriole disengagement is considered an essential step for licensing a new round of centriole duplication in the next cell cycle. Separase is critical for centriole disengagement. Here, we showed that pericentrin B (PCNTB) is specifically cleaved by separase at the exit of mitosis. The cleavage-resistant PCNTB mutant blocks the centriole disengagement and duplication. We also observed that an artificial cleavage of PCNTB during M phase induced premature disengagement of centrioles. Based on these results, we concluded that the separase-dependent cleavage of PCNTB is necessary and sufficient for centriole disengagement during mitosis.

摘要

中心体脱离被认为是在下一个细胞周期中许可新的一轮中心体复制的一个必要步骤。分离酶对于中心体脱离至关重要。在这里，我们表明，中心体旁蛋白 B（PCNTB）在有丝分裂结束时被分离酶特异性切割。不易被切割的 PCNTB 突变体阻止了中心体脱离和复制。我们还观察到，在 M 期人工切割 PCNTB 会诱导中心体过早脱离。基于这些结果，我们得出结论，分离酶依赖性的 PCNTB 切割对于有丝分裂期间中心体脱离是必要和充分的。