Renauer D, Gierlich H U, Unger K K
Institute of Toxicology, Johannes Gutenberg University, Mainz, Federal Republic of Germany.
Environ Health Perspect. 1990 Aug;88:243-6. doi: 10.1289/ehp.9088243.
High resolution HPLC techniques such as affinity chromatography (AC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) were used successfully for separations of hydrophobic plasma membrane glycoproteins. We have tested a lot of commercially available columns for IEC and SEC and performed the purification of the crude plasma membrane extract with the most suitable columns. By using immobilized ligands with different specificities and sequential affinity chromatography, it is possible to obtain a preliminary structural characterization of the interesting carbohydrate residues of membrane glycoproteins.
高分辨率高效液相色谱技术,如亲和色谱(AC)、离子交换色谱(IEC)和尺寸排阻色谱(SEC),已成功用于分离疏水性质膜糖蛋白。我们测试了许多市售的IEC和SEC柱,并使用最合适的柱对粗质膜提取物进行了纯化。通过使用具有不同特异性的固定化配体和顺序亲和色谱,可以对膜糖蛋白中有趣的碳水化合物残基进行初步的结构表征。