Irimura T, Nicolson G L
J Supramol Struct Cell Biochem. 1981;17(4):325-36. doi: 10.1002/jsscb.380170404.
The role of glycoconjugates in cell surface and blood-borne implantation properties of murine metastatic melanoma sublines of low (B16-F1) or high (B16-F10) potential to colonize lungs was investigated by treating melanoma cells with the antibiotic tunicamycin. This drug prevents glycosylation of glycoproteins by inhibiting the formation of lipid-linked oligosaccharide precursors. The degree of tunicamycin-mediated modifications in glycoproteins was assessed by monitoring the decrease in cell surface sialogalactoproteins by binding of 125I-labeled Ricinus communis agglutinin I. Scanning electron microscopy of tunicamycin-treated B16-F1 and B16-F10 cells showed morphologic changes such as cell rounding and formation of numerous surface blebs. Tunicamycin-treated B16-F1 and B16-F10 cells lost their lung colonization abilities when injected intravenously into C57BL/6 mice, concomitant with lowered rates of adhesion to endothelial cell monolayers, endothelial extracellular matrix (basal lamina), and polyvinyl-immobilized fibronectin in vitro, suggesting that this drug inhibits experimental metastasis by modifying the surface glycoproteins involved in determining the adhesive properties of malignant cells.
通过用抗生素衣霉素处理黑色素瘤细胞，研究了糖缀合物在低（B16 - F1）或高（B16 - F10）肺定植潜力的小鼠转移性黑色素瘤亚系的细胞表面和血行植入特性中的作用。这种药物通过抑制脂质连接寡糖前体的形成来阻止糖蛋白的糖基化。通过监测125I标记的蓖麻凝集素I的结合导致细胞表面唾液酸乳糖蛋白的减少，评估衣霉素介导的糖蛋白修饰程度。对经衣霉素处理的B16 - F1和B16 - F10细胞进行扫描电子显微镜检查，显示出形态学变化，如细胞变圆和形成许多表面泡。当经静脉注射到C57BL/6小鼠体内时，经衣霉素处理的B16 - F1和B16 - F10细胞失去了肺定植能力，同时在体外对内皮细胞单层、内皮细胞外基质（基膜）和聚乙烯固定的纤连蛋白的粘附率降低，这表明该药物通过修饰参与决定恶性细胞粘附特性的表面糖蛋白来抑制实验性转移。
