McCoy J P, Varani J, Goldstein I J
Anal Biochem. 1983 Apr 15;130(2):437-44. doi: 10.1016/0003-2697(83)90613-9.
Alkaline phosphatase has been coupled to Griffonia simplicifolia I B4 isolectin using a one-step glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound natural and synthetic glycoproteins bearing alpha-D-galactopyranosyl end groups. The extent of reactivity of the AP-GS I-B4 with the glycoproteins appears to be proportional to the number of terminal galactosyl residues present. Furthermore, this assay, termed ELLA (enzyme-linked lectin assay), is specifically inhibitable by low-molecular-weight sugars containing terminal alpha-D-galactosyl groups. The ELLA reactions may be assayed rapidly and objectively by the use of commercially available ELISA-plate using standard filters.
已使用一步戊二醛偶联程序将碱性磷酸酶与西非豆凝集素I B4同工凝集素偶联。这种酶-凝集素偶联物（AP-GS I-B4）已用于特异性检测带有α-D-吡喃半乳糖基端基的塑料结合的天然和合成糖蛋白。AP-GS I-B4与糖蛋白的反应程度似乎与存在的末端半乳糖基残基数量成正比。此外，这种称为ELLA（酶联凝集素测定）的测定可被含有末端α-D-半乳糖基的低分子量糖特异性抑制。ELLA反应可通过使用市售的ELISA板和标准滤光片快速客观地进行测定。
