Hild M, Böhnke M, Damms T, Heesemann J
Universitäts-Augenklinik, Universitätskrankenhaus, Hamburg, Bundesrepublik Deutschland.
Fortschr Ophthalmol. 1990;87(5):484-7.
Sera from 72 patients with uveitis and sera from 93 healthy blood donors were analyzed by an immunoblot technique for antibodies to plasmid-encoded virulence associated antigens of Yersinia enterocolitica. In the group of patients with acute anterior uveitis (n = 16), antibodies of the classes IgA, IgG, IgM were significantly increased in comparison to the healthy blood donors. The presence of IgA antibodies is a sign of acute or persistent Yersinia infection. For the other forms of uveitis these antibodies were not identifiable in such a significant number of patients. After 2 years, clinical and serological investigations were carried out again in these patients. At the time of the second investigation, 25/72 (34.7%) of these patients showed intraocular inflammatory activity (group 1), but in 47/72 (65.3%) ophthalmological inflammation was no longer present (group 2). The serological investigation of group 1 and group 2 showed no significant difference in the antibody response to Y. enterocolitica. From these results we conclude that a persistent or acute Yersinia infection is a possible trigger for acute anterior uveitis. To investigate an infection with Yersinia enterocolitica, a method demonstrating antibodies to the plasmid encoded antigens of Y. enterocolitica must be employed, because the virulence and the persistence of infection can only be analyzed by this technique.
采用免疫印迹技术，对72例葡萄膜炎患者的血清以及93名健康献血者的血清进行分析，以检测抗小肠结肠炎耶尔森菌质粒编码的毒力相关抗原的抗体。在急性前葡萄膜炎患者组（n = 16）中，与健康献血者相比，IgA、IgG、IgM类抗体显著增加。IgA抗体的存在是小肠结肠炎耶尔森菌急性或持续性感染的标志。对于其他类型的葡萄膜炎，在如此多的患者中未发现此类抗体。2年后，对这些患者再次进行临床和血清学检查。在第二次检查时，这些患者中有25/72（34.7%）表现出眼内炎症活动（第1组），但在47/72（65.3%）的患者中眼部炎症已不存在（第2组）。第1组和第2组的血清学检查显示，对小肠结肠炎耶尔森菌的抗体反应无显著差异。根据这些结果，我们得出结论，持续性或急性小肠结肠炎耶尔森菌感染可能是急性前葡萄膜炎的触发因素。为了研究小肠结肠炎耶尔森菌感染，必须采用一种能检测抗小肠结肠炎耶尔森菌质粒编码抗原抗体的方法，因为只有通过这种技术才能分析感染的毒力和持续性。
