Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universität München, Munich, Germany.
Cell. 2012 Jun 22;149(7):1431-7. doi: 10.1016/j.cell.2012.06.006.
We provide here a molecular movie that captures key aspects of RNA polymerase II initiation and elongation. To create the movie, we combined structural snapshots of the initiation-elongation transition and of elongation, including nucleotide addition, translocation, pausing, proofreading, backtracking, arrest, reactivation, and inhibition. The movie reveals open questions about the mechanism of transcription and provides a useful teaching tool.
我们在此提供一段分子电影，捕捉 RNA 聚合酶 II 起始和延伸的关键方面。为了创建这部电影，我们结合了起始延伸转变和延伸的结构快照，包括核苷酸的添加、移位、暂停、校对、回溯、停滞、重新激活和抑制。这部电影揭示了关于转录机制的悬而未决的问题，并提供了一个有用的教学工具。
