使用冷冻电子显微镜研究真核 RNA 聚合酶 I 的结构。
Structural Studies of Eukaryotic RNA Polymerase I Using Cryo-Electron Microscopy.
机构信息
Regensburg Center for Biochemistry (RCB), University of Regensburg, Regensburg, Germany.
出版信息
Methods Mol Biol. 2022;2533:71-80. doi: 10.1007/978-1-0716-2501-9_5.
Abstract
Technical advances have pushed the resolution limit of single-particle cryo-electron microscopy (cryo-EM) throughout the past decade and made the technique accessible to a wide range of samples. Among them, multisubunit DNA-dependent RNA polymerases (Pols) are a prominent example. This review aims at briefly summarizing the architecture and structural adaptations of Pol I, highlighting the importance of cryo-electron microscopy in determining the structures of transcription complexes.
摘要
技术的进步推动了单颗粒冷冻电子显微镜（cryo-EM）的分辨率极限在过去十年中不断提高，使得该技术能够应用于广泛的样品。其中，多亚基 DNA 依赖性 RNA 聚合酶（Pols）就是一个突出的例子。本文旨在简要总结 Pol I 的结构和结构适应性，强调 cryo-EM 在确定转录复合物结构方面的重要性。
相似文献
1
Structural Studies of Eukaryotic RNA Polymerase I Using Cryo-Electron Microscopy.使用冷冻电子显微镜研究真核 RNA 聚合酶 I 的结构。
Methods Mol Biol. 2022;2533:71-80. doi: 10.1007/978-1-0716-2501-9_5.
2
Preparation of RNA Polymerase Complexes for Their Analysis by Single-Particle Cryo-Electron Microscopy.用于单颗粒冷冻电子显微镜分析的 RNA 聚合酶复合物的制备。
Methods Mol Biol. 2022;2533:81-96. doi: 10.1007/978-1-0716-2501-9_6.
3
The cryo-EM resolution revolution and transcription complexes.冷冻电镜分辨率革命与转录复合物
Curr Opin Struct Biol. 2018 Oct;52:8-15. doi: 10.1016/j.sbi.2018.07.002. Epub 2018 Jul 14.
4
Structural insights into transcription initiation by yeast RNA polymerase I.酵母RNA聚合酶I转录起始的结构见解
EMBO J. 2017 Sep 15;36(18):2698-2709. doi: 10.15252/embj.201796958. Epub 2017 Jul 24.
5
Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes.痘病毒转录的结构基础：牛痘病毒 RNA 聚合酶复合物。
Cell. 2019 Dec 12;179(7):1537-1550.e19. doi: 10.1016/j.cell.2019.11.024.
6
7
The Structures of Eukaryotic Transcription Pre-initiation Complexes and Their Functional Implications.真核转录起始前复合物的结构及其功能意义
Subcell Biochem. 2019;93:143-192. doi: 10.1007/978-3-030-28151-9_5.
8
Cryo-EM in the study of challenging systems: the human transcription pre-initiation complex.冷冻电镜在具有挑战性的系统研究中的应用：人类转录起始前复合物
Curr Opin Struct Biol. 2016 Oct;40:120-127. doi: 10.1016/j.sbi.2016.09.009. Epub 2016 Sep 30.
9
Focused classification and refinement in high-resolution cryo-EM structural analysis of ribosome complexes.聚焦分类和细化在核糖体复合物的高分辨率冷冻电镜结构分析中。
Curr Opin Struct Biol. 2017 Oct;46:140-148. doi: 10.1016/j.sbi.2017.07.007. Epub 2017 Aug 29.
10
Structure of the initiation-competent RNA polymerase I and its implication for transcription.启动胜任 RNA 聚合酶 I 的结构及其对转录的影响。
Nat Commun. 2016 Jul 15;7:12126. doi: 10.1038/ncomms12126.
引用本文的文献
1
The ribosomal RNA transcription landscapes of Plasmodium falciparum and related apicomplexan parasites.恶性疟原虫及相关顶复门寄生虫的核糖体RNA转录图谱。
Nucleic Acids Res. 2025 Jul 8;53(13). doi: 10.1093/nar/gkaf641.
2
Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment.古菌 RNA 聚合酶转录延伸和 Spt4/5 募集的结构基础。
Nucleic Acids Res. 2024 Jun 10;52(10):6017-6035. doi: 10.1093/nar/gkae282.
本文引用的文献
1
Conserved strategies of RNA polymerase I hibernation and activation.RNA 聚合酶 I 休眠和激活的保守策略。
Nat Commun. 2021 Feb 3;12(1):758. doi: 10.1038/s41467-021-21031-8.
2
Structure of human RNA polymerase III.人类 RNA 聚合酶 III 的结构。
Nat Commun. 2020 Dec 17;11(1):6409. doi: 10.1038/s41467-020-20262-5.
3
DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability.基于 DNA 折纸术的单分子力谱解析 RNA 聚合酶 III 起始前复合物的稳定性。
Nat Commun. 2020 Jun 5;11(1):2828. doi: 10.1038/s41467-020-16702-x.
4
Structural basis of RNA polymerase I pre-initiation complex formation and promoter melting.RNA 聚合酶 I 起始前复合物形成和启动子解链的结构基础。
Nat Commun. 2020 Mar 5;11(1):1206. doi: 10.1038/s41467-020-15052-y.
5
RNA polymerase I (Pol I) passage through nucleosomes depends on Pol I subunits binding its lobe structure.RNA 聚合酶 I（Pol I）穿过核小体依赖于 Pol I 亚基与其叶状结构的结合。
J Biol Chem. 2020 Apr 10;295(15):4782-4795. doi: 10.1074/jbc.RA119.011827. Epub 2020 Feb 14.
6
Molecular insight into RNA polymerase I promoter recognition and promoter melting.RNA 聚合酶 I 启动子识别和启动子熔解的分子见解。
Nat Commun. 2019 Dec 5;10(1):5543. doi: 10.1038/s41467-019-13510-w.
7
8
Transcription initiation factor TBP: old friend new questions.转录起始因子 TBP：老朋友，新问题。
Biochem Soc Trans. 2019 Feb 28;47(1):411-423. doi: 10.1042/BST20180623. Epub 2019 Feb 1.
9
New tools for automated high-resolution cryo-EM structure determination in RELION-3.用于 RELION-3 中自动化高分辨率冷冻电镜结构测定的新工具。
Elife. 2018 Nov 9;7:e42166. doi: 10.7554/eLife.42166.
10
Structural basis of RNA polymerase I stalling at UV light-induced DNA damage.RNA 聚合酶 I 在 UV 光诱导的 DNA 损伤处停滞的结构基础。
Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):8972-8977. doi: 10.1073/pnas.1802626115. Epub 2018 Aug 20.
Zotero 插件