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利用新的 16S rDNA 特异性引物进行 DNA 扩增，灵敏而稳健地检测柑橘黄龙病菌“亚洲韧皮杆菌”。

Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers.

机构信息

National Institute of Fruit Tree Science, Fujimoto 2-1, Tsukuba, Ibaraki 305-8605, Japan.

出版信息

Mol Cell Probes. 2012 Oct;26(5):194-7. doi: 10.1016/j.mcp.2012.06.001. Epub 2012 Jun 21.

DOI:10.1016/j.mcp.2012.06.001
PMID:22728344
Abstract

Citrus greening disease is caused by "Candidatus Liberibacter spp.," including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR. More Las-infected tissues were detected with our primers than with the common primers.

摘要

黄龙病是由“韧皮部杆菌属”，包括“亚洲韧皮部杆菌（Las）”引起的。为了检测这种疾病，我们根据 Las 16S rDNA 设计了新的引物，并使用非常小的 DNA 模板进行 PCR。与常用引物相比，我们的引物检测到了更多感染 Las 的组织。

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