Wijaya Sumi, Jin Khoo Teng, Nee Ting Kang, Wiart Christophe
The University of Nottingham, Malaysia Campus.
J Complement Integr Med. 2012 Jun 20;9:Article 11. doi: 10.1515/1553-3840.1615.
The aim of this study was to investigate the antioxidant and anti-inflammatory potentials of crude extracts of Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm. and its isolated compounds. Separation and structure elucidation of Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm. were conducted using chromatographic and spectroscopic method. The antioxidant activities of the extracts in this study were determined by the ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching assays meanwhile the anti-inflammatory activity was evaluated using the 5-lipoxygenase assay. Seven known compounds quercetin 3-O-glucoside (1), naringenin 7-O-glucoside (2), seneciphylline-O-glucoside (3), chrysoeriol (4), retrorsine (5), adonifiline (6) and 5,4'-di-O-methyl alpinumisoflavone (7) were isolated from ethanol extract of Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm. The ethanol and water extracts of Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm. elicited potent antioxidant and anti-inflammatory properties. Amongst the isolated compounds quercetin 3-O-glucoside gave strong antioxidant activity and adonifiline strongly inhibited 5-lipoxygenase activity.
本研究的目的是调查皱叶洛菲莲（Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm.）粗提取物及其分离化合物的抗氧化和抗炎潜力。采用色谱和光谱方法对皱叶洛菲莲进行分离和结构解析。本研究中提取物的抗氧化活性通过铁还原抗氧化能力（FRAP）、2,2-二苯基-1-苦基肼（DPPH）和β-胡萝卜素漂白试验测定，同时使用5-脂氧合酶试验评估其抗炎活性。从皱叶洛菲莲（Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm.）的乙醇提取物中分离出七种已知化合物，即槲皮素3-O-葡萄糖苷（1）、柚皮素7-O-葡萄糖苷（2）、千里光叶碱-O-葡萄糖苷（3）、 Chrysoeriol（4）、倒千里光碱（5）、福寿草碱（6）和5,4'-二-O-甲基高山异黄酮（7）。皱叶洛菲莲的乙醇提取物和水提取物具有较强的抗氧化和抗炎特性。在分离出的化合物中，槲皮素3-O-葡萄糖苷具有较强的抗氧化活性，福寿草碱强烈抑制5-脂氧合酶活性。
