Departament of Obstetrics and Gynecology, Medical School, Universidad de Santiago de Chile, Santiago, Chile.
Braz J Infect Dis. 2012 May-Jun;16(3):273-8.
The role of mycoplasmas on the development and sequelae of pelvic inflammatory disease remains controversial. The objective of the present study is to correlate directly the presence of Mycoplasmateceae through polimerase chain reaction (PCR) determinations in cervix and Fallopian tubes of infertile patients with tubo-peritoneal factor diagnosed through laparoscopy.
Thirty patients with tubo-peritoneal infertility and 30 normal fertile patients were included in the study; cervical samples and tubal flushings were obtained during laparoscopy. PCR determinations for the detection of genetic material of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealiticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis in cervix and tubal flushings were performed.
No Mycoplasmataceae species as "only" microorganisms were found in tubal flushings of tubo-peritoneal infertility patients, whereas three (10%) fertile patients with normal tubes were positive for mycoplasma presence. This difference was not significant (p = 0.237). Among the 30 patients suffering from tubal infertility diagnosed through laparoscopy, Mycoplasmatecae species were not detected in the Fallopian tubes by PCR determinations, while in normal tubes from fertile patients these and other microorganisms could be found without distorting tubal anatomy.
Mycoplasmateceae species were not detected in Fallopian tubes of women with tubo-peritoneal infertility.
支原体在盆腔炎性疾病的发展和后遗症中的作用仍存在争议。本研究的目的是通过聚合酶链反应（PCR）直接检测不孕患者宫颈和输卵管中支原体科的存在，并将其与通过腹腔镜诊断的输卵管-腹膜因素不孕相关联。
本研究纳入了 30 例输卵管-腹膜因素不孕患者和 30 例正常生育患者；在腹腔镜检查期间获取宫颈标本和输卵管冲洗液。进行 PCR 检测以检测宫颈和输卵管冲洗液中生殖支原体、人型支原体、解脲脲原体、淋病奈瑟菌、沙眼衣原体和阴道毛滴虫的遗传物质。
在输卵管-腹膜因素不孕患者的输卵管冲洗液中未发现支原体科作为“唯一”微生物，而 30 例正常输卵管的生育患者中有 3 例（10%）存在支原体。这一差异无统计学意义（p=0.237）。在通过腹腔镜诊断为输卵管性不孕的 30 例患者中，PCR 检测未在输卵管中检测到支原体科，而在生育患者的正常输卵管中可以发现这些和其他微生物，而不会扭曲输卵管解剖结构。
在输卵管-腹膜因素不孕妇女的输卵管中未检测到支原体科。
