Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter.

Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO(2) NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda-Shimazaki et al., 2010. Int J Mol Sci 11:2383-2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter-reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO(2) NP response in some cell lines, although different sensor cells had different TiO(2) NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO(2) NP time-course response and dose response. The results indicated that our sensor cells can detect TiO(2) NP cellular responses. Our work should aid in understanding the interactions between bio-nanomaterials and cells.