Bhushan Ravi, Dixit Shuchi
Department of Chemistry, Indian Institute of Technology Roorkee, India.
Biomed Chromatogr. 2012 Aug;26(8):962-71. doi: 10.1002/bmc.2773. Epub 2012 Jun 25.
Amino acids are unique in terms of their structural features and multidimensional uses. With their simple structures and the ready availability of both enantiomers, amino acids not only serve as a chiral pool for synthesis but also provide an inexpensive pool for resolution studies. There has been no attempt to review the application of amino acids as chiral selectors for chromatographic enantioresolution of pharmaceuticals and other compounds. The present paper deals with application of l-amino acids and complexes of l-amino acids with a metal ion, particularly Cu(II), as an impregnating reagent in thin-layer chromatography or as a chiral ligand exchange reagent or a chiral mobile phase additive in both thin-layer chromatography and high-performance liquid chromatography. Enantiomeric resolution of β-blockers, nonsteroidal anti-inflammatories, amino acids (and their derivatives) and certain other compounds is discussed.
氨基酸在其结构特征和多方面用途上具有独特性。由于其结构简单且两种对映体都易于获得，氨基酸不仅作为合成的手性源，还为拆分研究提供了一个廉价的资源库。目前尚未有人尝试综述氨基酸作为手性选择剂在药物及其他化合物色谱对映体拆分中的应用。本文探讨了L-氨基酸及其与金属离子（特别是Cu(II)）的配合物作为浸渍试剂在薄层色谱中的应用，以及作为手性配体交换试剂或手性流动相添加剂在薄层色谱和高效液相色谱中的应用。文中还讨论了β-受体阻滞剂、非甾体抗炎药、氨基酸（及其衍生物）和某些其他化合物的对映体拆分情况。
