Bhushan R, Joshi S
Department of Chemistry, University of Roorkee, India.
Biomed Chromatogr. 1993 Sep-Oct;7(5):235-50. doi: 10.1002/bmc.1130070502.
Application of HPLC as a prime tool in the area of enantiomeric resolution has opened doors of success and varied interest. Use of chiral reagents either indirectly (as derivatization reagent) or directly (added to stationary or mobile phase) has led to achieve resolution of a wide range of compounds. Amino acids, being important molecules with simple structure and easy availability, have been extensively studied. A bibliographic survey on HPLC resolution of amino acids and derivatives along with a brief discussion on general methods of enantiomeric separation has been presented.
高效液相色谱法（HPLC）作为对映体拆分领域的主要工具，开启了成功之门并引发了广泛关注。使用手性试剂，无论是间接使用（作为衍生化试剂）还是直接使用（添加到固定相或流动相中），都已实现了多种化合物的拆分。氨基酸作为结构简单且易于获取的重要分子，已得到广泛研究。本文对氨基酸及其衍生物的HPLC拆分进行了文献综述，并简要讨论了对映体分离的一般方法。
