State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
J Chem Ecol. 2012 Aug;38(8):1003-16. doi: 10.1007/s10886-012-0152-4. Epub 2012 Jun 23.
Plant volatiles play a key role in host plant location of phytophagous insects. Cydia molesta is an important pest of pear fruit late in the growing season. We identified and quantified volatiles from immature and mature fruits of six pear varieties by using gas chromatography-mass spectrometry (GC-MS). Attractiveness of synthetic blends to adults based on gas chromatography-electroantennogram detection (GC-EAD) activity was investigated in both field and laboratory. Consistent electroantennographic activity was obtained for 12 compounds from headspace collections of the mature fruits of the six pear varieties. Qualitative and quantitative differences were found among six odor profiles. Among the six mixtures, the mixture of 1-hexanol, nonanal, ethyl butanoate, butyl acetate, ethyl hexanoate, hexyl acetate, hexyl butanoate, and farnesene (different isomers) with a 1:1:100:70:7:5:1:4 ratio from the variety Jimi and the mixture of nonanal, ethyl butanoate, 3-methylbutyl acetate, ethyl hexanoate, hexyl acetate, and farnesene with a 1:100:1:32:1:2 ratio from the variety Huangjin were highly attractive to both sexes in the field. However, male captures were much higher than those of females. Further wind tunnel tests proved that both sexes exhibited upwind flight to the lures, but only males landed on the source. Our finding indicates that mixtures mimicking Jimi and Huangjin volatiles attract both females and males of C. molesta, and these host volatiles may be involved in mate finding behavior.
植物挥发物在植食性昆虫对寄主植物的定位中起着关键作用。桃小食心虫是生长季节后期梨果的重要害虫。我们使用气相色谱-质谱联用仪（GC-MS）鉴定和量化了 6 个梨品种未成熟和成熟果实中的挥发物。根据气相色谱-触角电位检测（GC-EAD）活性，在田间和实验室中研究了基于合成混合物对成虫的吸引力。从 6 个梨品种成熟果实的顶空收集物中获得了 12 种化合物的一致触角电位活性。在 6 种气味图谱中发现了定性和定量差异。在这 6 种混合物中，混合物的 1-己醇、壬醛、丁酸乙酯、乙酸丁酯、己酸乙酯、乙酸己酯、己酸丁酯和法呢烯（不同异构体）与品种 Jimi 的比例为 1:1:100:70:7:5:1:4，混合物的壬醛、丁酸乙酯、3-甲基丁酸乙酯、己酸乙酯、乙酸己酯和法呢烯与品种 Huangjin 的比例为 1:100:1:32:1:2 对雌雄两性均具有较高的吸引力。然而，雄虫的捕获量远高于雌虫。进一步的风洞试验证明，雌雄两性均表现出顺风飞向诱饵，但只有雄虫降落在源头上。我们的发现表明，模仿 Jimi 和 Huangjin 挥发物的混合物吸引桃小食心虫的雌雄两性，这些寄主挥发物可能参与了求偶行为。
