Center for Biomaterials, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT 06030, USA.
Macromol Biosci. 2012 Aug;12(8):1034-42. doi: 10.1002/mabi.201200043. Epub 2012 Jun 25.
These studies provide evidence for the ability of a commercially available, defined, hyaluronan-gelatin hydrogel, HyStem-C™, to maintain both mouse embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs) in culture while retaining their growth and pluripotent characteristics. Growth curve and doubling time analysis show that mESCs and hiPSCs grow at similar rates on HyStem-C™ hydrogels and mouse embryonic fibroblasts and Matrigel™, respectively. Immunocytochemistry, flow cytometry, gene expression and karyotyping reveal that both human and murine pluripotent cells retain a high level of pluripotency on the hydrogels after multiple passages. The addition of fibronectin to HyStem-C™ enabled the attachment of hiPSCs in a xeno-free, fully defined medium.
这些研究为一种市售的、明确的透明质酸-明胶水凝胶 HyStem-C™ 的能力提供了证据，该水凝胶能够在保持其生长和多能性特征的同时，维持小鼠胚胎干细胞（mESCs）和人诱导多能干细胞（hiPSCs）的培养。生长曲线和倍增时间分析表明，mESCs 和 hiPSCs 在 HyStem-C™水凝胶上的生长速度与小鼠胚胎成纤维细胞和 Matrigel™分别相似。免疫细胞化学、流式细胞术、基因表达和核型分析表明，在多次传代后，两种多能细胞在水凝胶上均保持高水平的多能性。在 HyStem-C™中添加纤连蛋白使 hiPSCs 能够在无动物成分的、完全定义的培养基中附着。
